Skip to main content
. Author manuscript; available in PMC: 2020 Sep 4.
Published in final edited form as: Nature. 2020 Mar 4;579(7798):279–283. doi: 10.1038/s41586-020-2074-6

Figure 3.

Figure 3.

Chronic increases in mitochondrial oxidation with a continuous 3.5 week glucagon infusion reverse of hepatic steatosis and improve glucose tolerance in an InsP3R-I-dependent manner. (a)-(b) Liver VCS and VFAO with acute glucagon infusion (n=5 WT-glucagon, 6 KO-glucagon, 6 WT+glucagon, 5 KO+glucagon). (c) Liver TAG concentrations in HFD mice chronically infused with glucagon (n=9 WT-glucagon, 8 WT+glucagon, 8 KO-glucagon, 7 KO+glucagon). (d) Liver ceramide (n=6). (e) Liver DAG (n=9 -glucagon, 7 +glucagon). (f) PKCε translocation (n=6). (g)-(h) Plasma glucose and insulin during a glucose tolerance test (n=10 WT-glucagon, 11 WT+glucagon, 8 KO-glucagon, 8 KO+glucagon). In all panels, the mean±S.E.M. is shown. Groups were compared using the 2-tailed unpaired Student’s t-test.