Extended Data Figure 5.
Glucagon requires InsP3-mediated intrahepatic lipolysis to promote VPC and hepatic gluconeogenesis. (a) Body weight in mice treated with an adeno-associated virus to knock down liver ATGL (n=6). (b) Representative western blots. Blots from the same tissue (liver or WAT) were stripped and reprobed for all proteins shown. (c) WAT and liver ATGL protein expression (n=4, with the exception of KO+ATGL knockdown, in which n=6). (d)-(e) Hepatic PC and PEPCK protein expression (n=6 WT and KO+ATGL knockdown, in other groups, n=4). (f)-(h) Plasma glucagon, NEFA and glycerol concentrations in mice treated with an adeno-associated virus to knock down ATGL in a liver-specific manner (n=6, with the exception of WT+ATGL knockdown and KO, in which n=5). Groups were compared before and after glucagon by the 2-tailed unpaired Student’s t-test. (i)-(j) Liver glycogen and malonyl-CoA content (n=6 other than WT+ATGL knockdown, in which n=5). All comparisons were performed using the 2-tailed unpaired Student’s t-test, unless otherwise stated. If no statistical comparison is denoted, the groups were not significantly different. Error bars represent the S.E.M.