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. 2020 Feb 27;5(4):e131494. doi: 10.1172/jci.insight.131494

Figure 5. Dlx1–/– adults have normal neuronal and glial numbers.

Figure 5

(A–X) Representative images and quantifications of adult Dlx1–/– mouse proximal small intestine (SI), distal SI, mid-colon, and distal colon immunostained using HuC/D (magenta) and TuJ1 (green) antibodies. Myenteric plexus (A–H) and submucosal plexus (M–T) are shown. Neuronal density was normal in Dlx1–/– mice for all bowel regions analyzed (I–L, U–X; Student’s t test; n ≥ 3 per condition). (Y and Z) Adult Dlx1–/– distal SI was immunostained using an antibody against SOX10, which exclusively labels glia in adults (red). (AA) Quantitative analysis of glial density shows no difference in glial cell density in distal SI of Dlx1–/– mice or WT mice (Student’s t test; n = 3 per condition). Scale bars: 100 μm.