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. 2020 Feb 27;5(4):e132094. doi: 10.1172/jci.insight.132094

Figure 6. Adenosine-like compounds restore LND-relevant neuronal phenotypes.

Figure 6

(A–C) Evaluation of hit compound efficacy upon cell viability (A, Hoechst-PI staining readout), percentage of Ki-67+ cells (B), and total cell number (C) after treating LND neural cells repeatedly with 2.0 μM azaserine from day 5 of differentiation. The results represent the mean ± SD of LND1 and LND2 lines, with 4 technical replicates/donor, compared with 0.1% DMSO-treated cells. (D and E) Evaluation of hit compound efficacy, as assessed through the percentage (E) and total number (F) of HuC/D+ neurons after treating LND neural cells repeatedly with 2.0 μM azaserine from day 5 of differentiation. The results represent the mean ± SD of LND1 and LND2 lines, with 4 technical replicates/donor, compared with 0.1% DMSO-treated cells. (F) Representative images of immunocytochemistry for Hoechst (blue), Ki-67, and HuC/D staining (green) on day 7 of differentiation. Scale bar: 100 μm. Aza, azaserine.