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. 2020 Feb 27;5(4):e125937. doi: 10.1172/jci.insight.125937

Figure 3. fpr1–/– mice are not protected from adeno-TGF-β1–induced lung fibrosis.

Figure 3

(A) C57BL/6 and fpr1–/– mice were challenged intratracheally with 4 × 108 PFUs of TGF-β1 adenovirus (Ad-TGF-β1223/225) or control virus (Ad-DL) in 30 μl sterile saline and lung tissue–harvested on day 21. (B) Representative α-smooth muscle actin–stained (αSMA-stained) and Picrosirius red–stained lung tissue on day 21. (C) Hydroxyproline (μg/lobe) content of lung tissue on days 5 and 21. (D) Percentage area positive of αSMA and (E) Picrosirius red staining on day 21. Data represent the mean value of 20 randomly selected, nonoverlapping fields (original magnification, ×20). Relative gene expression of TGF-β1 (F), αSMA (G), Collagen 1a1 (H), Collagen 1a2 (I), and TIMP1 (J) in lung tissue on day 21 was assessed by qPCR. Gene expression was normalized to GAPDH as a loading control. n = 6–8 mice per group. No significant difference was seen between control virus–treated C57BL/6 and fpr1–/– mice, and therefore control virus mice were pooled and presented as mean (red-hashed line). Data were analyzed using a Mann-Whitney U test and presented as box-and-whisker plots. All P > 0.05.