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. 2020 Mar 10;9:e50988. doi: 10.7554/eLife.50988

Table 1. Analysis of tomographic data sets used throughout this study.

Full data sets Partial data sets
Spindle parameters Metaphase no. 1 Metaphase no. 2 Metaphase no. 3 Anaphase onset Anaphase no. 1 Anaphase no. 3 Anaphase no. 4 Anaphase no. 7 Anaphase no. 2 Anaphase no. 5 Anaphase no. 6
MTs total 1729 2406 1689 2051 1405 1540 1403 1881 (893) (671) (246)
MTs within 150 nm from chromosomes (KMTs) 524 912 650 794 633 821 752 944 (580) (499) (160)
End-on associated KMTs on X-chromosome 29 38 38 53 27 50 38 42 57 30 43
Lateral associated KMTs on X-chromosome 79 34 91 22 61 55 33 34 47 52 28
End-on associated KMTs on autosomes 154 355 199 318 106 189 181 175
Lateral associated KMTs on autosomes 262 485 321 400 437 527 500 692
Autosome-to-autosome distance [µm] 0.76 0.94 0.99 1.00 2.98 3.35 3.37 5.44 (3.14) (3.58) (4.47)
Autosomes1-to-X distance [µm] 0.37 0.43 0.43 0.47 1.45 1.45 1.37 1.95 (1.54) (1.71)
Autosomes2-to-X distance [µm] 0.39 0.51 0.56 0.54 1.56 2.02 1.99 3.51 (1.72) (1.90)
Pole-to-pole distance [µm] 3.10 3.41 3.45 3.51 4.97 5.22 4.99 7.04
Pole1-to-X distance [µm] 1.64 1.66 1.66 1.62 2.60 2.29 2.21 2.69
Pole2-to-X distance [µm] 1.48 1.76 1.80 1.90 2.39 3.04 2.77 4.38
Autosome-to-centrosome distance [µm] 1.18 1.24 1.23 1.26 1.00 0.94 0.82 0.83
Mother-to-daughter centriole distance [µm] 0.26 0.21 0.27 0.35 0.37 0.73 0.74 1.11
Original name of data set T0391_worm13 metaphase01 T0391_worm14 metaphase T0391_worm13 metaphase02 T0391_worm13 meta-anaphase01 T0391_worm05 anaphase02 T0391_worm08 lateanaphase T0391_anaphase01 early T0391_worm09 late_anaphase T0391_worm07b T0391_worm06 T0391_worm02
Number of sections 14 11 14 25 14 17 11 30 8 4 6
Est. tomographic volume [µm³] 102.33 94.70 107.52 115.12 113.96 131.92 101.51 268.31 36.99 18.52 30.37

The table summarizes all microtubule numbers and distances as measured within the electron tomographic reconstructions in this study. A kinetochore microtubule (KMT) is defined as a microtubule that is at least 150 nm from the surface of a chromosome. KMTs are sub-divided into end-on and lateral associated MTs. End-on KMTs are defined as pointing towards the chromosome surface, lateral MTs are all remaining KMTs. Distances were measured between the geometric centers of autosomes (mean position of individual autosomes), centrosomes (center point of both centrioles) and centrioles (between the centers of the mother and daughter centriole). Tomographic volumes were estimated by multiplying the X-Y dimensions of each tomogram with the number of sections (with a section thickness of 300 nm).