Table 1. Analysis of tomographic data sets used throughout this study.
Full data sets | Partial data sets | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Spindle parameters | Metaphase no. 1 | Metaphase no. 2 | Metaphase no. 3 | Anaphase onset | Anaphase no. 1 | Anaphase no. 3 | Anaphase no. 4 | Anaphase no. 7 | Anaphase no. 2 | Anaphase no. 5 | Anaphase no. 6 |
MTs total | 1729 | 2406 | 1689 | 2051 | 1405 | 1540 | 1403 | 1881 | (893) | (671) | (246) |
MTs within 150 nm from chromosomes (KMTs) | 524 | 912 | 650 | 794 | 633 | 821 | 752 | 944 | (580) | (499) | (160) |
End-on associated KMTs on X-chromosome | 29 | 38 | 38 | 53 | 27 | 50 | 38 | 42 | 57 | 30 | 43 |
Lateral associated KMTs on X-chromosome | 79 | 34 | 91 | 22 | 61 | 55 | 33 | 34 | 47 | 52 | 28 |
End-on associated KMTs on autosomes | 154 | 355 | 199 | 318 | 106 | 189 | 181 | 175 | |||
Lateral associated KMTs on autosomes | 262 | 485 | 321 | 400 | 437 | 527 | 500 | 692 | |||
Autosome-to-autosome distance [µm] | 0.76 | 0.94 | 0.99 | 1.00 | 2.98 | 3.35 | 3.37 | 5.44 | (3.14) | (3.58) | (4.47) |
Autosomes1-to-X distance [µm] | 0.37 | 0.43 | 0.43 | 0.47 | 1.45 | 1.45 | 1.37 | 1.95 | (1.54) | (1.71) | |
Autosomes2-to-X distance [µm] | 0.39 | 0.51 | 0.56 | 0.54 | 1.56 | 2.02 | 1.99 | 3.51 | (1.72) | (1.90) | |
Pole-to-pole distance [µm] | 3.10 | 3.41 | 3.45 | 3.51 | 4.97 | 5.22 | 4.99 | 7.04 | |||
Pole1-to-X distance [µm] | 1.64 | 1.66 | 1.66 | 1.62 | 2.60 | 2.29 | 2.21 | 2.69 | |||
Pole2-to-X distance [µm] | 1.48 | 1.76 | 1.80 | 1.90 | 2.39 | 3.04 | 2.77 | 4.38 | |||
Autosome-to-centrosome distance [µm] | 1.18 | 1.24 | 1.23 | 1.26 | 1.00 | 0.94 | 0.82 | 0.83 | |||
Mother-to-daughter centriole distance [µm] | 0.26 | 0.21 | 0.27 | 0.35 | 0.37 | 0.73 | 0.74 | 1.11 | |||
Original name of data set | T0391_worm13 metaphase01 | T0391_worm14 metaphase | T0391_worm13 metaphase02 | T0391_worm13 meta-anaphase01 | T0391_worm05 anaphase02 | T0391_worm08 lateanaphase | T0391_anaphase01 early | T0391_worm09 late_anaphase | T0391_worm07b | T0391_worm06 | T0391_worm02 |
Number of sections | 14 | 11 | 14 | 25 | 14 | 17 | 11 | 30 | 8 | 4 | 6 |
Est. tomographic volume [µm³] | 102.33 | 94.70 | 107.52 | 115.12 | 113.96 | 131.92 | 101.51 | 268.31 | 36.99 | 18.52 | 30.37 |
The table summarizes all microtubule numbers and distances as measured within the electron tomographic reconstructions in this study. A kinetochore microtubule (KMT) is defined as a microtubule that is at least 150 nm from the surface of a chromosome. KMTs are sub-divided into end-on and lateral associated MTs. End-on KMTs are defined as pointing towards the chromosome surface, lateral MTs are all remaining KMTs. Distances were measured between the geometric centers of autosomes (mean position of individual autosomes), centrosomes (center point of both centrioles) and centrioles (between the centers of the mother and daughter centriole). Tomographic volumes were estimated by multiplying the X-Y dimensions of each tomogram with the number of sections (with a section thickness of 300 nm).