Table 2. Measurements of spindle dynamics in male meiosis.
Distance | Spindle parameter | Meiosis I | Meiosis II | ||
---|---|---|---|---|---|
Mean | SD | Mean | SD | ||
P-P1 | Initial spindle length (metaphase) | 4.1 µm | ±0.3 µm | 4.2 µm | ±0.4 µm |
Final spindle length (end of anaphase) | 8.0 µm | ±0.6 µm | 8.8 µm | ±0.8 µm | |
Initial rate (1 st minute) | 1.29 μm/min | ±0.36 μm/min | 1.11 μm/min | ±0.42 μm/min | |
Duration of elongation | 3–4 min | 8–9 min | |||
A-A2 | Initial spindle length (metaphase) | 0.9 µm | ±0.2 µm | 0.6 µm | ±0.2 µm |
Final spindle length (end of anaphase) | 6.5 µm | ±0.4 µm | 8.0 µm | ±0.8 µm | |
Initial rate (1 st minute) | 2.07 µm/min | ±0.37 μm/min | 2.16 µm/min | ±0.32 μm/min | |
Duration of elongation | 4–5 min | 8–9 min | |||
P-A3 | Initial spindle length (metaphase) | 1.6 µm | ±0.3 µm | 2.0 µm | ±0.4 µm |
Final spindle length (end of anaphase) | 0.8 µm | ±0.3 µm | 0.4 µm | ±0.2 µm | |
Initial rate (1 st minute) | −0.39 µm/min | ±0.27 μm/min | −0.64 µm/min | ±0.33 μm/min |
Distances: 1P-P, pole-to-pole distance; 2A-A, autosome-to-autosome distance; 3P-A, pole-to-autosome distance. Initial spindle length is given at metaphase, final distance refers to the end of anaphase when spindle elongation plateaus. Values are given as mean values (± standard deviation, SD). The numbers of analyzed spindles are: n = 31 for meiosis I; n = 50 for meiosis II.