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Extending ligation sites beyond Cys through a) Ser/Thr ligation, b) NCL followed by Cys alkylation, c) NCL followed by desulfurization (not limited to examples of β-thiol amino acids shown – we direct the reader to the following reviews323, 324), d) traceless-NCL using removable ligation auxiliaries,20 e) NCL at selenocysteine followed by selective deselenization (not limited to examples shown – we direct the reader to the following reviews324, 325).
