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Semisynthesis of PSD-95 PDZ domains. a) Semisyntheses of phosphorylated PDZ1–3. b) Semisynthesis of PSD-95 PDZ2 with amide-to-ester mutants at the substrate binding site. Right: Important H-bonding interactions in the PDZ-substrate complex backbone, mapped onto a canonical PDZ-substrate structure (PDB ID: 1BE9).229
