Skip to main content
. Author manuscript; available in PMC: 2021 Mar 25.
Published in final edited form as: Chem Rev. 2019 Nov 27;120(6):3051–3126. doi: 10.1021/acs.chemrev.9b00450

Figure 8.

Figure 8.

Production of recombinant N-terminal cysteinyl proteins through proteolysis of fusion proteins in vitro. a) Commonly used proteases for the generation of N-terminal cysteinyl proteins for NCL. b) Preparation of recombinant cFos leucine zipper domain with an N-terminal cysteine for NCL (Erlanson et al.).28 c) Preparation of recombinant N-terminal cysteinyl truncated H3 for the semisynthesis of H3K9me3 (Nguyen et al.).77 Inset: Structure of the Smt3/Ulp1 complex (PDB ID code 1EUV).