. 2020 Mar 27;10:5658. doi: 10.1038/s41598-020-62604-9

Table 2.

Primers used in this work.

Primer name	Sequence 5′-3′	Product
∆gE-F	ATACTGCCGGCCAGACTACGGAACCTCAACAATTGGTACGTAGGGATAACAGGGTAATCGATTT	Kana gene flanked by homology arms of gE
∆gE-R	TAACTATTTCACTAGTGAGTCATTAGTTCAACATCCATGACGTACCAATTGTTGAGGTTCCGTAGTCTGGCCGGCAGTATGCCAGTGTTACAACCAAT	Kana gene flanked by homology arms of gE
RgE-F	TTGGAGTACTAAACACCAACATACTGCCGGCCAGACTACGGAACCTCAACAATTGGTACGATGATGGTTACTTTTATATC	gE gene flanked by homology arms of gE
RgE-R	TGAGTCATTAGTTCAACATCCATGATCAGATGCGGAAACTAGATT	gE gene flanked by homology arms of gE
RgE-Kana-F	TCATGGATGTTGAACTAATGACTCACTAGTGAAATAGTTACCTGTATTACTAGGGATAACAGGGTAATCGAT	Kana gene flanked by homology arms of gE
RgE-Kana-R	CAGGTGTCGGCCTAATATACCTGTGCATTAGTAATACAGGTAACTATTTCACTAGTGAGTCATTAGTTCAACATCCATGATGTTACAACCAATTAACCA	Kana gene flanked by homology arms of gE
gE-F	TCTCAAGACGCTCTGGAATC	gE gene identification primers
gE-R	AGCGAGTACTTCTCTGCGTC	gE gene identification primers