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. 2020 Mar 27;6:15. doi: 10.1038/s41522-020-0125-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Bacterial clumping of overnight cultures of esp-negative E. faecalis 23 and S. aureus Δbap grown under shaken conditions (200 rpm) at 37 °C in the presence of rEsp_N. Ø indicates no addition of rEsp_N to bacterial culture. b Inmunofluorescence showing surface localization of rEsp_N. Bacteria were fixed and labeled with anti-His antibodies and DAPI. Scale bars represent 3 µm. c Scanning electron microscopy and d transmission electron micrographs of negatively stained E. faecalis 23 incubated with (right panel) and without rEsp_N (left panel). Scale bar represents 500 nm.