Table 3.

Primers.

Primer	Sequence
Esp_Ori_Bam	5′-GGATCCAGGAAGGGAGCAAATTTAGTTTGT-3′
Esp_B_Kpn	5′-GGTACCATTTTTACTTACAGTTACTGCTAAATCG-3′
K-3xF-ClfA	5′-GGTACCGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGGAAATTGAACCAATTCCAGAGGAT-3’
ClfA-7cE	5′-TTACACCCTATTTTTTCGCC-3′
EF1-InPhu-pCN51-BamH	5′-CGACTCTAGAGGATCCAATAAGGAAGGGAGCAAATTTAGTTTG-3′
Esp2-InPhu-pCN51-KpnI	5′-CTGAATTCGAGCTCGGTACGGAAGAGACTTCTTCCTCTTTTCAGA-3′
EspB-NotI-5	5′-GCGGCCGCACAAGAAAATCCGGGATATACG-3′
EspB-XhoI-3	5′-CTCGAGTTAGTGATGATGGTGATGGTGTGCTAATACAGTACCTTCTTTATCAAC-3′
Esp-Lic-Fw	5′-GACGACGACAAGATGCAAATGGGTGAAG-3′
Esp-Lic-Rv	5′-GAGGAGAAGCCCGGTTAACTGTAAGTAAAAAT-3′