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. 2020 Mar 27;10:5576. doi: 10.1038/s41598-020-62476-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sample collection details for female and male D. magna. Sample ages for females are shown at the top where the minimum sampled age is 10 days and the maximum sampled age is 80 days. Male sample ages are shown on the bottom and have a minimum sampled age of 8 days and maximum sample age of 40 days. Ages shown in red have a biological replicate number of 8, ages shown in black have a biological replicate number of 2.