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. 2020 Mar 27;10:5576. doi: 10.1038/s41598-020-62476-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Principal component analysis of normalised positive and negative ionisation mode lipidomics data for male and female Daphnia magna demonstrated as percentage of lifespan. Principal component 1 shows clear separation based on sex (F = female, M = male) with females grouped to the left and males to the right. Principal component 2 shows separation by age depicted by colour ranging from orange (young) to blue (old). Female D. magna age range is 10 days to 80 days (maximum sampled age = 80 days). For female age groups 20, 40 and 80 days, eight technical replicates were used and for intermediate ages two technical replicates were used. Male D. magna age groups ranging from 8 days to 40 days were used (maximum sampled age = 40 days). For male D. magna age groups 10, 20 and 40 days, eight technical replicates were used and for intermediate age groups two technical replicates were used. For detailed information regarding biological replicate numbers see Supplementary Tables S1 and S2. Supplementary Figures S5 and S6 show PCA plots for positive and negative ion mode separately and highlight the grouping of the intrastudy QC sample.