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. 2020 Mar 27;10:5576. doi: 10.1038/s41598-020-62476-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The Venn diagram represents an overview of the number of lipids with significant change in their relative intensity between categories of sex, age and sex:age interaction, and ageing in males and females in D. magna. (a) The number of annotated lipids found to have a statistically-significant change in relative intensity (adjusted p-value <0.001) across the factors sex, age, and sex:age interaction are represented by numbers and the respective percentage within each grouping. The 505 lipids outside of the groupings do not show significantly (adjusted p-value <0.001) changing relative intensities in any of the conditions presented. (b) The number of lipids identified as showing a significant change in relative intensity (adjusted p-value <0.001) observed within the conditions of ageF (female ageing data only) and ageM (male ageing data only) are represented by numbers and percentages within each grouping.