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. 2020 Mar 27;10:5576. doi: 10.1038/s41598-020-62476-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Venn diagram of lipids statistically-significantly changing lipid intensity with age between increment age groups compared to youngest age group for each gender. (a) Comparison of lipids in female D. magna showing statistically-significant changes in lipid intensity (p-adjusted <0.001) from age groups 20, 40 and 80 days compared to youngest female group aged 10 days shows larger difference with increased age (e.g. between 10 and 20 days only 87 unique lipids identified showing similarity between age groups compared to 340 unique lipids between aged 10 days and 80 days). The size of the circle is proportionally relative to number of lipids. (b) Comparison of significant lipids in male D. magna (p-adjusted <0.001) from age groups 10, 20 and 40 days compared to youngest male group aged 8 days shows larger difference with increased age (e.g. between 8 and 10 days only 34 unique lipids identified showing similarity between age groups compared to 548 unique lipids between aged 8 days and 40 days).