Figure 6.

Directional change of lipid levels significantly changing in (a) female and (b) male D. magna. Stacked normalised bar chart shows percentage of lipid classes that are changing with age in D. magna. Lipids showing overall decline with age are shown in blue whilst lipids showing overall increase with age are shown in red. Grey represents no significant change with age (adjusted p-value <0.001). Numbers on the x-axis represent the total number of lipids identified in each class, for lipid class abbreviation descriptions and classification see Supplementary Table S7. For female age groups 20, 40 and 80 days eight technical replicates were used and for intermediate age groups two technical replicates were used. For detailed information regarding biological replicate numbers see Supplementary Table S1. For male age groups 10, 20 and 40 days eight technical replicates were used and for intermediate age groups two technical replicates were used. For detailed information regarding biological replicate numbers see Supplementary Table S2.