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. 2020 Mar 27;10:5576. doi: 10.1038/s41598-020-62476-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lipid class changes in sex:age interaction in male and female D. magna. Stacked normalised bar chart shows percentage of lipid classes that are changing with sex:age interaction. A faster rate of change in males with age is shown in blue whilst slower rate in males with age (faster rate in females) is shown in red. Grey represents no significant change with interaction between sex and age (adjusted p-value <0.001)). For female age groups 20, 40 and 80 days eight technical replicates were used and for intermediate age groups two technical replicates were. For male D. magna age groups 10, 20 and 40 days eight technical replicates were used and for intermediate age groups two technical replicates were used. For detailed information regarding biological replicate numbers see Supplementary Tables S1 and S2. Numbers on the x-axis represent the total number of lipids identified in each class. Numbers on the x-axis represent the total number of lipids identified in each class, for lipid class abbreviation descriptions and classification see Supplementary Table S7.