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. 2020 Mar 27;10:5576. doi: 10.1038/s41598-020-62476-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heatmap of lipid changes with sex:age interaction. Heatmap of lipidomics analysis for top 50 lipids significantly-changing concentrations in positive ion mode (dark purple) and top 50 lipids significantly changing levels in negative ion mode (light purple, adjusted p-value <0.001) in D. magna. The heat map shows 100 selected lipids indicated by class colours (rows) and 29 samples (columns). Colours correspond to per-lipid z-score that is computed from area under the curve. Lipids and samples were hierarchically clustered based on Euclidean cluster extraction and average linkage. Numbers on the x-axis represent the total number of lipids identified in each class, for lipid class abbreviation descriptions and classification see Supplementary Table S7.