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. 2020 Mar 27;10:5580. doi: 10.1038/s41598-020-62619-2

Table 1.

Sequences of Quantitative Real-Time PCR primers.

Gene	Primers	Reaction conditions
PKR	Fw:5′-CCAGGCAAACAAGGTCCCATC-3′	95 °C 15 sec 60 °C 30 sec 72 °C 20 sec	35 cycles
PKR	Rev: 5′-GCGAGTGTGCTGGTCACTAA-3′	95 °C 15 sec 60 °C 30 sec 72 °C 20 sec	35 cycles
18S rRNA	Fw: 5′-CTCAACACGGGAAACCTCAC-3′	95 °C 15 sec 60 °C 30 sec 72 °C 35 sec	35 cycles
18S rRNA	Rev: 5′-CGCTCCACCAACTAAGAACG -3′	95 °C 15 sec 60 °C 30 sec 72 °C 35 sec	35 cycles
VHS	Fw: 5′-ACATAACTGCGGTGCTCTTC-3′	95 °C 15 sec 60 °C 30 sec 72 °C 45 sec	40 cycles
VHS	Rev: 5′-CCGAAATTCTAACCCAACAG-3′	95 °C 15 sec 60 °C 30 sec 72 °C 45 sec	40 cycles
US3	Fw: 5′-ACTGGCATGGGCTTTACGATC-3′	95 °C 15 sec 60 °C 30 sec 72 °C 45 sec	40 cycles
US3	Rev:5′-GGAGGACCAGACACGTGACCC-3′	95 °C 15 sec 60 °C 30 sec 72 °C 45 sec	40 cycles