Fig. 4.

Acacetin effect on the concentration of inflammatory cytokines in the bronchoalveolar lavage fluid. Twenty-four hours after sepsis induction by CLP, the lungs of the sepsis mice were flushed with saline and the bronchoalveolar lavage fluid (BALF) was collected. The concentration of the inflammatory cytokines tumor necrosis factor alpha (TNF-α,) (a), interleukin- (IL) 1β (b), IL-6 (c), and macrophage inflammatory protein-2 (MIP-2), (d) was determined using an ELISA assay. The values represent the mean ± SD (n = 10/group). ^*P < 0.05, compared to the non-treated control group; ^#P < 0.05, compared to the sepsis-induced ALI group