Fig. 5.

Acacetin effect on iNOS and COX-2 expression. Twenty-four hours after sepsis induction by CLP, sepsis mice in the different treatment groups were exsanguinated and their lung tissues were removed. The protein level of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in the lung homogenates was determined using Western blotting (Individual sample in each group was used in WB data and the combined group was used in bar graphs). a The protein level of iNOS in the lung tissues from mice in the different treatment groups. b The protein level of COX-2 in the lung tissues from mice in the different treatment groups. c The level of nitrite in the lung homogenates. d The level of prostaglandin E2 (PGE2) in the lung homogenates. The values represent the mean ± SD (n = 10/group). ^*P < 0.05, compared to the non-treated control group; ^#P < 0.05, compared to the sepsis-induced ALI group