Table 2.

Nomenclature, mass, and amino acid sequence of major exogenous His-apelin-55 processing products observed after incubation in HEK293A cell or 3T3-L1 adipocyte culture medium

Name	Mass (Da)	Amino acid sequence*
His-apelin-55	8380	SGSHHHHHHGSSGENLYFQSGSLMPLPDGNGLEDGNVRHLVQPRGSRNGPGPWQGGRRKFRRQRPRLSHKGPMPF
His-apelin-55Δ1	8233	SGSHHHHHHGSSGENLYFQSGSLMPLPDGNGLEDGNVRHLVQPRGSRNGPGPWQGGRRKFRRQRPRLSHKGPMP
His-apelin-55Δ15	6335	SGSHHHHHHGSSGENLYFQSGSLMPLPDGNGLEDGNVRHLVQPRGSRNGPGPWQGGRRKF
His-apelin-55Δ32	4202	SGSHHHHHHGSSGENLYFQSGSLMPLPDGNGLEDGNVRHLVQP
His-apelin-55Δ36	4196	SGSHHHHHHGSSGENLYFQSGSLMPLPDGNGLEDGNVRH

*Underlined residues represent N-terminal His₆ tag and TEV protease cleavage site