Table 4.
Western blot analysis of ACE2 protein levels and real-time RT-PCR analysis of the mRNA expression of RAS components from atrial tissues
| Sham | AF-Control | Ad-EGFP | Ad-ACE2 | p* | p # | p ♂ | |
|---|---|---|---|---|---|---|---|
| mRNA ACE2 | 1.00 ± 0.00 | 0.53 ± 0.28 | 0.51 ± 0.12 | 5.43 ± 0.77 | 0.042 | 0.034 | <0.001 |
| mRNA AT1R | 1.00 ± 0.00 | 1.54 ± 0.40 | 1.58 ± 0.09 | 0.98 ± 0.26 | <0.001 | <0.001 | <0.001 |
| mRNA AT2R | 1.00 ± 0.00 | 0.65 ± 0.22 | 0.60 ± 0.18 | 1.28 ± 0.55 | 0.048 | 0.026 | <0.001 |
| Protein ACE2 | 0.94 ± 0.27 | 0.56 ± 0.18 | 0.41 ± 0.19 | 1.84 ± 0.47 | 0.028 | 0.003 | <0.001 |
| Protein Mas | 0.80 ± 0.24 | 0.47 ± 0.18 | 0.50 ± 0.20 | 1.06 ± 0.39 | 0.032 | 0.048 | <0.001 |
* Sham vs. AF-control; # Sham vs. Ad-EGFP; ♂ Ad-ACE2 vs. Ad-EGFP (or AF-control), n = 7