Fig. 7.

Effect of NCSC-CM on the expression of nuclear NF-κB p65 and cytoplasmic IκB-β in the lung tissues. Treatment with NCSC-CM, but not heated NCSC-CM, increased IκB-β levels (a) and reduced nuclear NF-κB p65 levels (b) in ischemia-reperfusion (IR)-induced lung injury. PCNA and β-actin served as loading controls for nuclear and cytoplasmic proteins, respectively. A representative blot is shown. Data are expressed as mean ± SD (n = 3 per group). *P < 0.05, compared to the control group; #P < 0.05, compared to the IR group; +P < 0.05, compared to the IR+ NCSC-CM group.