Fig. 6.

Polygalasaponin F inhibited signaling pathway proteins expression in lung of mice infected with IAV by western blot analysis (n = 6). GAPDH protein was used as a loading control. a Representative PAGE of cell-signaling pathway proteins expression by western blotting. b Semi-quantitative analysis of IκBα protein. c Semi-quantitative analysis of NF-κB p65 protein. d Semi-quantitative analysis of MEK1 protein. Values are mean ± SD. Asterisks denote the significance levels: *p < 0.05; **p < 0.01; ***p < 0.001, compared with IAV-C group