Fig. 2.

The frequency of Treg cells and Tfr cells in the mini-BAL from ARDS patients at day 1, day 2, and day 3 after disease onset. a Fresh lymphocytes from mini-BAL were collected and examined using flow cytometry immediately following isolation. Figures shown were pre-gated on CD4⁺ T cells from day 1, day 2, and day 3 of the same ARDS patient. Treg cells were gated as total Foxp3⁺ CD4⁺ T cells, and Tfr cells were gated as Foxp3⁺CXCR5⁺ CD4⁺ T cells. b The MFI of Foxp3 in Tfr cells and non-Tfr Treg cells from the mini-BAL. Unpaired t test with Welch’s correction. c The frequencies of Treg cells, Tfr cells, and non-Tfr Treg cells in the mini-BAL from ten ARDS patients. RM 1-way ANOVA followed by Tukey’s test. *P < 0.05. **P < 0.01. ***P < 0.001. NS not significant