. 2020 Mar 15;2020:8475246. doi: 10.1155/2020/8475246

Table 2.

PCR thermo cycling conditions for primary and secondary PCR (for PF3D7_1343700).

Primer name	PCR	Sequence 5′-3′	Product	Initial denaturation	Denaturation	Annealing	Extension	Final extension	No. of cycles
K13_PCR-F	1⁰ PCR	CGGAGTGACCAAATCTGGGA	2097 bp	95-15 m	95-00.30 s	58-2.00 m	72-2.00 m	72-10.00 m	25
K13_PCR_R	1⁰ PCR	GGGAATCTGGTGGTAACAGC	2097 bp			58-2.00 m	72-2.00 m		25
K13_N1_F	2⁰ PCR	GCCAAGCTGCCATTCATTTG	849 bp			60-1.00 m	72-2.00 m		28
K13_N1_R	2⁰ PCR	GCCTTGTTGAAAGAAGCAGA	849 bp			60-1.00 m	72-2.00 m		28