Table 1. Strains and plasmids used in this study.
| Strains or plasmids | Relevant genotype | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| WT | E. coli isolated from a dairy cow with mastitis (ECDCM2), wild-type | Laboratory stock |
| XL4 | ECDCM2 qseBC-deletion mutant | This study |
| WT/pSTV28 | WT with the empty vector pSTV28, Cmra | This study |
| XL4/pSTV28 | XL4 with the empty vector pSTV28, Cmr | This study |
| XL4/pCqseBC | XL4 with the complement plasmid pCqseBC, Cmr | This study |
| Plasmids | ||
| pKD46 | Expresses λ Red recombinase Exo, Bet and Gam, temperature sensitive, Ampr | Datsenko & Wanner (2000) |
| pKD3 | cat gene, template plasmid, Ampr Cmr | Datsenko & Wanner (2000) |
| pCP20 | FLP+λcI857+λpRRep (Ts), temperature sensitive, Ampr Cmr | Datsenko & Wanner (2000) |
| pSTV28 | Low copy number cloning vector, Cmr | Takara |
| pCqseBC | pSTV28 with qseBC gene, Cmr | This study |
Notes.
a
Cmr, chloramphenicol-resistant; Ampr, ampicillin-resistant.