Table 2. Oligonucleotide primers used in this study.
| Primer name | Oligonucleotide sequence (5′–3′) |
|---|---|
| qseBC-f | GGATTTAACCTTACCAGGCA |
| qseBC-r | GAACCATCATCGCATGTGTG |
| knockout-qseBC-f | AGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCTGT AGGCTGGAGCTGCTT |
| knockout-qseBC-r | AGACGAGTAGCGCGATCGATCCCGGAATGTAATTGGAGCA TGAATATCCTCCTTAGTTC |
| check-qseBC-f | GCGTTACAACACGGTTTACT |
| check-qseBC-r | CGGTACGGTGAAATTAGCAA |
| Cm-f | TGTAGGCTGGAGCTGCTT |
| Cm-r | CATATGAATATCCTCCTTAGTTC |
| qseBC-EcoRI-f | CGGAATTCATGCGAATTTTACTGATAGA |
| qseBC-KpnI-r | GGGGTACCTTACCAGCTTACCTTCGTCT |
| M13-f | TGTAAAACGACGGCCAGT |
| M13-r | CAGGAAACAGCTATGACC |
| T7-f | TAATACGACTCACTATAGGG |
| T7-r | TGCTAGTTATTGCTCAGCGG |
| qseB-EcoR I | CCGGAATTCATGCGAATTTTACTGATAG |
| qseB-Hind III | CCCAAGCTTTCATTTCTCACCTAATGT |
| rt-16S-f | TTTGAGTTCCCGGCC |
| rt-16S-r | CGGCCGCAAGGTTAA |
| rt-bcsA-f | GATGGTACAAATCTTCCGTC |
| rt-bcsA-r | ATCTTGGAGTTGGTCAGGCT |
| rt-csgA-f | AGCGCTCTGGCAGGTGTTGT |
| rt-csgA-r | GCCACGTTGGGTCAGATCGA |
| rt-fliC-f | CCTGAACAACACCACTACCA |
| rt-fliC-r | TGCTGGATAATCTGCGCTTT |
| rt-motA-f | GGCAATAATGGCAAAGCGAT |
| rt-motA-r | CAGCGAAAACATCCCCATCT |
| rt-wcaF-f | TCTCGGTGCCGAAAGGGTTC |
| rt-wcaF-r | ATTGACGTCATCGCCGACCC |
| rt-fimA-f | TGCTGTCGGTTTTAACATTC |
| rt-fimA-r | ACCAACGTTTGTTGCGCTA |
| rt-acrA-f | GCAGCCAATATCGCGCAA |
| rt-acrA-r | ATGCGACCGCTAATCGGA |
| rt-acrB-f | TTGCCAAAGGCGATCACG |
| rt-acrB-r | TTGGCAGACGCACGAACA |
| rt-acrD-f | TGTTCCTGCGTTTGCCGA |
| rt-acrD-r | CATTCGCGCCACGTTTTG |
| rt-marA-f | TGTCCAGACGCAATACTG |
| rt-marA-r | TACGGCTGCGGATGTATT |
| rt-emrD-f | GTATTACTCGTGGCCGTC |
| rt- emrD-r | ATTCCGACGAGGATCACC |
| rt-mdtH-f | GCGAGGAACCTGGGTAAA |
| rt-mdtH-r | CCGCCGAAAATACCCAGA |
| Biotin-p-qseB-f | GTTTATTACTCCCTTTAATG |
| p-qseB-r | TTTTTCATCCCTGCGATAAC |
| Biotin-p-marA-f | TGGTGGTTGTTATCCTGTG |
| p-marA-r | ATTAGTTGCCCTGGCAAGT |
| Biotin-p-acrA-f | ATGTTCGTGAATTTACAGG |
| p-acrA-r | ATGTAAACCTCGAGTGTC |
| Biotin-p-acrD-f | TGCCTCCTACTGACCAAAGAA |
| p-acrD-r | TAAAAGAGGACCTCGTGTTTC |
| Biotin-p-emrD-f | CCGCTTTTGTTTACATAT |
| p-emrD-r | TATCACGGATGCTTTTAT |
| Biotin-p-mdtH-f | TTCCCCTCCCGGGAAATAAA |
| p-mdtH-r | TCTATACCTACTCCTTCCCG |
Notes.
a
The sequences with the underline refer to the restriction endonuclease recognition sites.
- f
- forward
- r
- reverse