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Transcription of pIFITM1–3 in response to PRV-GFP infection. A–E RT-qPCR analysis of IFN-β (B), IL-1β (C), pIFITM1 (D), pIFITM2 (E) and pIFITM3 (F) mRNA expression in mock-infected or PRV-GFP (MOI 0.1) infected PK15 and 3D4/21 cells at 0, 3, 6, 12 or 24 h post-infection. All data are shown as mean ± SD based on three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 determined by a two-tailed Student’s t-test.
