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PRV-induced pIFITM1 expression is dependent on cGAS, STING, TBK1, IRF3 and IFNAR1. A–E RT-qPCR analysis of pIFITM1 mRNA in WT, cGAS−/− (A), STING−/− (B), TBK1−/− (C), IRF3−/− (D) and IFNAR1−/− (E) PK15 cells infected with PRV-QXX (MOI = 1) at 0, 2, 4, 6, 12 or 24 h post-infection. Data are shown as mean ± SD based on three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 determined by a two-tailed Student’s t-test. ns, no significance.
