Fig. 2.

Expression of ZE3 N-RICs and C-RICs. (A) To test whether crude leaf extracts of the ZE3 C-RIC and N-RIC constructs were stable upon acid-precipitation, 1 N phosphoric acid was added to a final acid volume of 3% of the total soluble extract. Following a five-minute incubation, the samples were neutralized with 2 M Tris base. Samples of extract without acid-precipitation were included for comparison. The samples were mixed with non-reducing sample buffer and loaded on a 4–15% polyacrylamide gel. The Western blot was probed with HRP-labeled goat anti-human IgG (H + L). Abbreviations: Minus (-) refers to leaf extract without acid precipitation, and plus (+) refers to leaf extract after acid-precipitation. (B) Leaves were infiltrated with ZE3 C-RIC or N-RIC expression vectors and crude leaf extracts were analyzed for IgG production by ELISA at 5 DPI. Columns represent mean ± the standard deviation of 4 independently infiltrated leaf samples. The p value was measured by student’s t-test.