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Purification of ZE3 VLPs. After sucrose gradient sedimentation, the 30–50% sucrose layers for (A) HBche-ZE3 or (B) HBc-ZE3 were pooled, dialyzed, and analyzed by reducing SDS-PAGE gel stained with Coomassie stain (left lane) and western blot (right lane) probed with a polyclonal rabbit anti-Zika envelope antibody. Electron microscopy of dialyzed peak sucrose fractions from gradient after negative staining with 0.5% uranyl acetate; bar = 100 nm.
