REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Monoclonal anti-HA antibody produced in mouse	Sigma-Aldrich	Cat.#: H3663; RRID: AB_262051
Monoclonal anti-β-actin antibody produced in mouse	Sigma-Aldrich	Cat.#: A5441; RRID: AB_476744
Monoclonal anti-VSV-M (23H12) antibody	KeraFast	Cat.#: EB0011; RRID: AB_2734773
Polyclonal anti-ACE2 antibody	R&D Systems	Cat.#: AF933; RRID: AB_355722
Polyclonal anti-DC-SIGN antibody	Santa Cruz	Cat.#: sc-11038; RRID:AB_639038
Monoclonal anti-mouse, peroxidase-coupled	Dianova	Cat.#: 115-035-003; RRID:AB_10015289
Anti-VSV-G antibody (I1, produced from CRL-2700 mouse hybridoma cells)	ATCC	Cat.# CRL-2700; RRID:CVCL_G654
Bacterial and Virus Strains
VSV∗ΔG-FLuc	(Berger Rentsch and Zimmer, 2011)	N/A
SARS-CoV-2 isolate Munich 929	Laboratory of Christian Drosten	N/A
One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli	ThermoFisher Scientific	Cat.#: C854003
Biological Samples
Patient serum, CSS-2	Laboratory of Christian Drosten	N/A
Patient serum, CSS-3	Laboratory of Andreas Nitsche	N/A
Patient serum, CSS-4	Laboratory of Andreas Nitsche	N/A
Patient serum, CSS-5	Laboratory of Andreas Nitsche	N/A
Rabbit serum, anti-SARS-S1 rabbit I	Laboratory of Stefan Pöhlmann	N/A
Rabbit serum, anti-SARS-S1 rabbit II	Laboratory of Stefan Pöhlmann	N/A
Chemicals, Peptides, and Recombinant Proteins
Camostat mesylate	Sigma-Aldrich	SML0057
E-64d	Sigma-Aldrich	E8640
Ammonium chloride	Carl Roth	Cat.#: 5050.2
Critical Commercial Assays
Beetle-Juice Kit	PJK	Cat.#: 102511
CellTiter-Glo® Luminescent Cell Viability Assay	Promega	Cat.#: G7570
Experimental Models: Cell Lines
A549	Laboratory of Georg Herrler	ATCC Cat# CRM-CCL-185; RRID:CVCL_0023
BEAS-2B	Laboratory of Stefan Pöhlmann	ATCC Cat# CRL-9609; RRID:CVCL_0168
Calu-3	Laboratory of Stephan Ludwig	ATCC Cat# HTB-55; RRID:CVCL_0609
NCI-H1299	Laboratory of Stefan Pöhlmann	ATCC Cat# CRL-5803; RRID:CVCL_0060
Huh-7	Laboratory of Thomas Pietschmann	JCRB Cat# JCRB0403; RRID:CVCL_0336
Caco-2	Laboratory of Stefan Pöhlmann	ATCC Cat# HTB-37; RRID:CVCL_0025
Vero	Laboratory of Andrea Maisner	ATCC Cat# CRL-1586; RRID:CVCL_0574
Vero-TMPRSS2	This paper	N/A
LLC-PK1	Laboratory of Georg Herrler	ATCC Cat# CRL-1392; RRID:CVCL_0391
MDBK	Laboratory of Georg Herrler	ATCC Cat# CCL-22; RRID:CVCL_0421
MDCKII	Laboratory of Georg Herrler	ATCC Cat# CRL-2936; RRID:CVCL_B034
RhiLu/1.1	Laboratory of Christian Drosten, Laboratory of Marcel A. Müller	N/A; RRID: CVCL_RX22
MyDauLu/47.1	Laboratory of Christian Drosten, Laboratory of Marcel A. Müller	N/A; RRID: CVCL_RX18
BHK-21	Laboratory of Georg Herrler	ATCC Cat# CCL-10; RRID:CVCL_1915
NIH/3T3	Laboratory of Stefan Pöhlmann	ATCC Cat# CRL-1658; RRID:CVCL_0594
HAE	HTCR Foundation (Human Tissue and Cell Research)	N/A
293T	DSMZ	Cat.#: ACC-635; RRID: CVCL_0063
Oligonucleotides
SARS-2-S (BamHI) F AAGGCCGGATCCGCCACCATGTTTCT GCTGACCACCAAGC	Sigma-Aldrich	N/A
SARS-2-S (XbaI) R AAGGCCTCTAGATTAGGTGTAGTGCAG TTTCACG	Sigma-Aldrich	N/A
SARS-2-S-HA (XbaI) R AAGGCCTCTAGATTACGCATAATCC GGCACATCATACGGATAGGTGTAGTGCAGTTTCACG	Sigma-Aldrich	N/A
WH-Ssyn 651F CAAGATCTACAGCAAGCACACC	Sigma-Aldrich	N/A
WH-Ssyn 1380F GTCGGCGGCAACTACAATTAC	Sigma-Aldrich	N/A
WH-Ssyn 1992F CTGTCTGATCGGAGCCGAGCAC	Sigma-Aldrich	N/A
WH-Ssyn 2648F TGAGATGATCGCCCAGTACAC	Sigma-Aldrich	N/A
WH-Ssyn 3286F GCCATCTGCCACGACGGCAAAG	Sigma-Aldrich	N/A
Recombinant DNA
Synthetic, codon-optimized (humanized) SARS-2-S	ThermoFisher Scientific (GeneArt)	N/A
Plasmid: pCG1-SARS-S	(Hoffmann et al., 2013)	N/A
Plasmid:pCG1-SARS-S-HA	This paper	N/A
Plasmid: pCG1-SARS-2-S	This paper	N/A
Plasmid: pCG1-SARS-2-S-HA	This paper	N/A
Plasmid: pCAGGS-229E-S	(Hofmann et al., 2005)	N/A
Plasmid: pCAGGS-MERS-S	(Gierer et al., 2013)	N/A
Plasmid: pCAGGS-VSV-G	(Brinkmann et al., 2017)	N/A
Plasmid: pCAGGS-NiV-F	Laboratory of Andrea Maisner	N/A
Plasmid: pCAGGS-NiV-G	Laboratory of Andrea Maisner	N/A
Plasmid: pCG1-hACE2	(Hoffmann et al., 2013)	N/A
Plasmid: pCG1-batACE2	(Hoffmann et al., 2013)	N/A
Plasmid: pCG1-hAPN	(Hofmann et al., 2004a)	N/A
Plasmid: pQCXIP-DsRed-hDPP4	(Kleine-Weber et al., 2018)	N/A
Plasmid: pQCXIBL-hTMPRSS2	(Kleine-Weber et al., 2018)	N/A
Plasmid: pCG1	Laboratory of Roberto Cattaneo	N/A
Plasmid: pCAGGS-DsRed	Laboratory of Stefan Pöhlmann	N/A
Plasmid: pCAGGS-eGFP	Laboratory of Stefan Pöhlmann	N/A
Software and Algorithms
Hidex Sense Microplate Reader Software	Hidex Deutschland Vertrieb GmbH	https://www.hidex.de/
ChemoStar Imager Software (version v.0.3.23)	Intas Science Imaging Instruments GmbH	https://www.intas.de/
MEGA 7.0.26	Kumar et al., 2018	https://www.megasoftware.net
Adobe Photoshop CS5 Extended (version 12.0 × 32)	Adobe	https://www.adobe.com/
GraphPad Prism (version 8.3.0(538))	GraphPad Software	https://www.graphpad.com/
Microsoft Office Standard 2010 (version 14.0.7232.5000)	Microsoft Corporation	https://products.office.com/