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. 2020 Jan 21;39(4):379–388. doi: 10.1016/j.healun.2019.12.009

Figure 2.

Figure 2

Exosomes containing lung-associated SAgs in LTxRs diagnosed with RVI and stable LTxRs. Exosomes isolated from serum samples of LTxRs with RVI (n = 34) and stable LTxRs (n = 30) were analyzed for the presence of lung-associated SAgs (Col-V and Kα1T) by immunoblot. The mean relative optical densities of Col-V (1.9 ± 0.2 vs 0.73 ± 0.09, p = 0.0003) and Kα1T (4.06 ± 1.09 vs 0.83 ± 0.31, p = 0.009) were significantly higher in LTxRs with RVI than stable LTxRs. Optical density was measured using ImageJ software and the OD value of SAgs were calculated in LTxRs with RVI and stable LTxRs after normalization with CD9 OD value. CD9 also served as loading control and exosome-specific markers. The presence of lung SAgs in the exosomes was compared between the cohorts using Mann–Whitney test. Asterisk indicates statistically significant. Col-V, collagen-V; Kα1T, K-alpha-1 tubulin; LTxR, lung transplant recipient; OD, optical density; RVI, respiratory viral infection; SAg, self-antigen.