Fig. 8.

Confirmation of three differentially expressed proteins (SP, AP and Spn-2) with Western blotting. A, The protein levels of SP, AP and Spn-2 in P50−, P50+, A35− and A35+. 30 μg each of midgut digestive juice protein was analyzed by western blot with antiserum (top). Total proteins were Coomassie Brilliant Blue (CBB)-stained as the loading control (bottom). B, Label-free results from 3 DEPs in P50 and A35 strains showing BmNPV-infected and control groups. C, Densitometric intensity of the Western blot was analyzed by using Image J software. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)