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. Author manuscript; available in PMC: 2021 Jan 9.
Published in final edited form as: Cell. 2019 Dec 26;180(1):150–164.e15. doi: 10.1016/j.cell.2019.12.004

Figure 3. RIXC localizes across heterochromatin domains and at boundary elements.

Figure 3.

(A) Immunopurified fractions from the indicated strains were subjected to mass spectrometry. The total peptide coverage (%) for the identified proteins is shown. (B) Co-IP of GFP-tagged RIXC with TAP-Swi6. Anti-Swi6 antibody was used to detect endogenous Swi6 (Input, lane 1), TAP-tagged (Input, lane 2) and TEV-cleaved TAP-Swi6 (IP lanes). (C and D) ChIP-chip (C) and ChIP-qPCR (mean ± SD, n=3) (D) showing enrichment of GFP-Rix1 and Las1-GFP at heterochromatic loci. (E) Schematic (top) indicating the positions of B-boxes (red arrows) in the IR-R element. ChIP-seq analysis of GFP-Rix1 in the indicated strains. These strains harbor IR-LΔ to assess IR-R-specific enrichments. The B-boxΔ cells lack a 240-bp sequence that contains 5 B-boxes. (F) Schematic illustrating Swi6-independent and -dependent recruitment of RIXC at mat. See also Figure S3.