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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Lab Invest. 2019 Dec 19;100(4):516–526. doi: 10.1038/s41374-019-0354-2

Figure 4.

Figure 4.

Airway hyperresponsiveness to antigen, lung inflammation and airway remodeling develop in an OVA-induced model of chronic asthma elicited in BALB/c-KitW-sh/W-sh mice. A. Penh values were measured 1 h before and 1, 3, 6 and 17 h after the eighth PBS or OVA challenge. B. Leukocyte numbers in bronchoalveolar lavage fluid (BALF) were determined by manual counting and by differential counts on cytospin slides. C. Histology in H&E-stained sections of lungs. Solid arrowheads indicate perivascular inflammation, and solid arrows indicate peribronchiolar inflammation. D. Numbers of infiltrating leukocytes quantified per mm2 of airway. E. PAS stain of mouse airways. Scale bars in C & E indicate 100 μm. F. Goblet cell numbers per mm of airway basement membrane (BM). G. Plasma titers of OVA-specific IgE and IgG1 as determined by ELISA. Results are expressed as arbitrary units. For Penh measurements and BAL cell counts, for OVA-sensitized and challenged mice (OVA mice), N=9 for BALB/c-KitW-sh/W-sh and N=5 for BALB/c-Kit+/+ mice; for PBS mock-sensitized and challenged mice (PBS mice), N=4 for BALB/c-KitW-sh/W-sh and N=3 for BALB/c-Kit+/+ mice. For goblet cell counts, N=5 for BALB/c-KitW-sh/W-sh OVA and N=4 for BALB/c-Kit+/+ OVA mice, and N=4 for BALB/c-KitW-sh/W-sh PBS and N=3 for BALB/c-Kit+/+ PBS mice. For OVA-specific antibody levels, N=8 for BALB/c-KitW-sh/W-sh OVA, N=4 for BALB/c-Kit+/+ OVA, N=4 for BALB/c-KitW-sh/W-sh PBS, and N=3 for BALB/c-Kit+/+ PBS. A, B, F & G: Data were pooled from age-matched mice from 3 separate experiments, each of which gave similar results. Mean values are shown ± s.e.m. (A) or + s.e.m. (B, D, F & G). Similar results were obtained from a second group of age-matched older mice. For Penh measurements, ** p<0.01 for BALB/c-Kit+/+ OVA vs. BALB/c-Kit+/+ PBS, * p<0.02 for BALB/c-KitW-sh/W-sh OVA vs. BALB/c-KitW-sh/W-sh PBS as determined by 2-way ANOVA. For BALF cell counts and infiltrating leukocytes counts on histology, p values for t tests comparing BALB/c-Kit+/+ PBS vs. BALB/c-Kit+/+ OVA, BALB/c-KitW-sh/W-sh PBS vs. BALB/c-KitW-sh/W-sh OVA, and BALB/c-Kit+/+ OVA vs. BALB/c-KitW-sh/W-sh OVA that remained significant after correction for performing multiple t tests are shown (see Methods). For goblet cells counts, group means were compared by ANOVA. Mean antibody titers were compared by ANOVA.