Extended Data Fig. 2. Nuclear defects are intrinsic to Lmna KO myonuclei.

(a) Top, schematic of the generation of hybrid myofibers containing nuclei from both Lmna WT and Lmna KO cell lines. Bottom, corresponding representative images. Final hybrid fibers contained ~80% Lmna WT nuclei and 20% Lmna KO nuclei. Arrowheads denote Lmna KO nucleus with a chromatin protrusion residing within the same myofiber as a Lmna WT nucleus. Experiments were conducted three independent times, with similar results. (b) Quantification of the number of chromatin protrusions from Lmna WT and Lmna KO nuclei contained within isogenic myofibers (control) or hybrid myofibers, typically containing 80% Lmna WT and 20% Lmna KO nuclei. Data points are for n independent experiments, in which 91–163 nuclei were quantified per experiment. Significance determined by two-way ANOVA (nuclear genotype vs. isogenic or hybrid myofiber), using Tukey’s correction for multiple comparisons. All bar plots show mean value ± standard error of the mean.