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. Author manuscript; available in PMC: 2020 Jun 16.
Published in final edited form as: Nat Mater. 2019 Dec 16;19(4):464–473. doi: 10.1038/s41563-019-0563-5

Extended Data Fig. 3. Nuclear envelope rupture is increased in Lmna N195K myofibers in vitro and in vivo.

Extended Data Fig. 3

Quantification of cGAS-mCherry nuclear envelope rupture reporter foci formation during myoblast differentiation in Lmna N195K (a), Lmna H222P (b), Lmna cells and wild-type littermate controls (Lmna WT). n = 3 independent experiments per genotype. (c) Quantification of the percentage of myonuclei positive for cGAS-tdTomato foci in isolated muscle fibers from Lmna WT, Lmna KO, Lmna N195K, and Lmna H222P mice expressing the cGAS-tdTomato transgene. Analysis performed for whole fiber (left) and by classification of nuclei located at the MTJ or within the body of the fiber (right). Data for Lmna WT and Lmna KO reproduced from Fig. 4E for comparison. Data based on n individual myofibers per genotype, isolated from 4–5 animals each.. All bar plots show mean value ± standard error of the mean.