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. 2020 Jan 28;48(6):3228–3243. doi: 10.1093/nar/gkz1240

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Rescuing ‘impaired’ spacers. (A) Cleavage efficiency is shown in terms of fluorescence loss for various crRNA variants. The mature repeat is shown in blue, the 1–19 nt of spacer sequence in red, the 20–24 nt positions are shown in yellow. The crRNAs are categorised as ‘impaired’ and ‘rescued’ either with (pTarget7) or without (pTarget8) a 3′ terminator sequence. Average values from three biological replicates are shown, with error bars representing SD. (B) Detail of the structures in (A).