Table 3.

List of modified sequences studied and their thermal stability

Name	Type of study	Sequence (5′-3′)	T m (°C)	ΔTm (°C)
AT26	Original	TGG TGG TGG TGG TTG TGG TGG TGG TTG T	40.8 ± 0.5	-
insT27	Modifications of edgewise loop	TGG TGG TGG TGG TTG TGG TGG TGG TTTG T	44.0 ± 0.5	+3.2
insT27, 28		TGG TGG TGG TGG TTG TGG TGG TGG TTTTG T	35.7 ± 1.2	-5.1
delT13	Modifications of V-shaped loop	TGG TGG TGG TGG -TG TGG TGG TGG TTG T	NAa	NAa
insT15		TGG TGG TGG TGG TTTG TGG TGG TGG TTG T	37.6 ± 0.7	-3.2
insT15,16		TGG TGG TGG TGG TTTTG TGG TGG TGG TTG T	34.0 ± 0.8	-6.8
delT4	Deletion of bulges	TGG -GG TGG TGG TTG TGG TGG TGG TTG T	57.0 ± 0.0	+16.2
delT16		TGG TGG TGG TGG TTG -GG TGG TGG TTG T	50.6 ± 0.3	+ 9.8
delT4,16		TGG -GG TGG TGG TTG -GG TGG TGG TTG T	66.2 ± 0.5	+25.4
insT9	Addition of bulges	TGG TGG TGTG TGG TTG TGG TGG TGG TTG T	NAa	NAa
insT9,22		TGG TGG TGTG TGG TTG TGG TGTG TGG TTG T	NAa	NAa
AT26E	G-tetrad extension	TGG -GG TGG TGG TTG -GGG TGGG TGGG TTGG	>90	>+49

^aNMR spectra indicate the formation of multiple G-quadruplex conformations.