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. 2020 Feb 12;48(6):3181–3194. doi: 10.1093/nar/gkaa093

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — OSGEPL1 is a monomer and does not interact with YrdC. (A) Determination of the molecular mass of OSGEPL1 based on protein standards and elution volumes. (B) OSGEPL1-FLAG and OSGEPL1-Myc were co-expressed in HEK293T cells, and OSGEPL1-Myc could not be pulled down by OSGEPL1-FLAG in a Co-IP assay. (C) OSGEPL1-FLAG could not be precipitated by OSGEPL1-Myc in a Co-IP assay. (D) Dimeric Qri7 was used as a positive control. Qri7-FLAG and Qri7-Myc were co-expressed in HEK293T cells, and Qri7-Myc could be pulled down by Qri7-FLAG in a Co-IP assay. (E) Dimeric hmThrRS was used as a positive control, and hmThrRS-Myc was pulled down by hmThrRS-FLAG, as expected. (F) OSGEPL1-FLAG and YrdC-HA were co-expressed in HEK293T cells, and YrdC-HA could not be pulled down by OSGEPL1-FLAG in a Co-IP assay. (G) OSGEPL1-FLAG could not be precipitated by YrdC-HA in a Co-IP assay.