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. 2020 Feb 12;48(6):3181–3194. doi: 10.1093/nar/gkaa093

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The hmtRNA^Thr transcript is the best substrate of YrdC/OSGEPL1 in vitro. (A) Time-course curves of the modification of five hmtRNAs, hmtRNA^Asn (green triangles), hmtRNA^Ile (red squares), hmtRNA^Lys (blue diamonds), hmtRNA^Ser(AGY) (pink hexagons) and hmtRNA^Thr (black filled circles), by YrdC and OSGEPL1. (B) t⁶A modification at increasing concentrations of hmtRNA^Asn (10 μM, red filled squares; 20 μM, green filled triangles; 40 μM, blue filled inverted triangles and 80 μM, pink filled diamonds). A control without tRNA addition (no tRNA, orange circles) was included. (C) LC–MS/MS analysis of the digestion product of the hmtRNA^Thr transcript in the absence and presence of standard t⁶A or modified hmtRNA^Thr transcripts by YrdC/OSGEPL1 or Sua5/Qri7.