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. 2020 Feb 12;48(6):3181–3194. doi: 10.1093/nar/gkaa093

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Nucleotide requirements in the anticodon loop. (A) Mutagenesis of hmtRNA^Thr at the non-Watson-Crick base pair (A29-C41; left), and mutagenesis of hmtRNA^Met at the anticodon loop base (C34 and C38; right) are coloured red. (B) t⁶A modification levels of hmtRNA^Thr (black filled circles) and its mutants C32A (red filled squares), C32U (magenta filled triangles) and C32G (blue filled inverted triangles). (C) t⁶A modification levels of hmtRNA^Thr (black filled circles) and mutants U33A (red filled squares), U33C (cyanine filled triangles) and U33G (blue filled inverted triangles). (D) t⁶A modification levels of hmtRNA^Thr (black filled circles) and mutants U34A (red filled squares), U34C (cyanine filled triangles) and U34G (blue filled inverted triangles). (E) t⁶A modification levels of hmtRNA^Thr (black filled circles) and mutants G35A (red filled squares), G35C (cyanine filled triangles) and G35U (magenta filled inverted triangles). (F) t⁶A modification levels of hmtRNA^Met (black filled circles) and mutants C34U (red filled squares), C38A (green filled triangles) and C34U/ C38A (magenta filled inverted triangles).