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. 2020 Feb 12;48(6):3181–3194. doi: 10.1093/nar/gkaa093

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — MTS-deleted OSGEPL1 can rescue loss of Kae1 in vivo. (A) Genes encoding Kae1, Qri7, Qri7-ΔMTS, OSGEPL1, OSGEPL1-ΔMTS and OSGEP were transformed into ScΔKae1, transformants were spread on SD/Leu⁻ or SD/Leu⁻/5-FOA plates, and the growth phenotype was observed. Kae1 and p425TEF empty vectors were used as positive and negative controls, respectively. (B) Yeast growth curves were determined after 5-FOA selection in SD/Leu⁻ liquid culture at an initial cell density (OD₆₀₀) of 0.03.