Figure 1.

Survey of WDR5 binding sites in six different cell lines. (A) Results of ChIP-Seq analyses of WDR5 in six cell lines, showing (top row) the number of WDR5 peaks, and (bottom row) the number of genes assigned to those peaks in each line. (B) Heat maps of WDR5 ChIP-Seq peak intensity in each line. The figure represents the combined average of normalized peak intensity in 100-bp bins ± 2 kb around the center of peaks. For mouse cells, the heatmap presents the combined peaks in the two mouse cell lines; for human cells, the heatmap presents the combined peaks in the four human cell lines. Peaks are ranked based on MC-38 for mouse cells and LoVo for human cells. (C) Immunoblotting of steady-state WDR5 levels in the indicated cell lines. GAPDH is a loading control. (D) Distribution of WDR5 binding sites, relative to annotated TSS. ChIP-Seq peaks for all six cell lines are plotted according to distance from nearest TSS, binned by region: ±0–500, 500–1000, 1000–5000 and >5000 bp away from TSS. (E) Averaged TSS-proximal ChIP-Seq peak shape and distribution in all six cell lines. Fragment depth is normalized to the maximum peak read for each cell line. (F) Results of known-motif analysis of WDR5 ChIP-Seq peaks, showing the top-ranked E-box motif for each line. p-value, motif name, and percent of target sequences containing the motif are listed beneath each motif sequence. (G) GO enrichment analysis of WDR5-bound genes in each cell line. The top five conserved GO categories for each line are shown; numbers to the right of each bar show the number of genes in each category.