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. 2020 Feb 13;48(6):3366–3378. doi: 10.1093/nar/gkaa083

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Analysis of the interaction of TetR and TetR mutant variants TetR-Q38A and TetR-R28A with either the RNA aptamer or tetO operator-encoding DNA. (A) EMSA showing the interaction of the RNA aptamer with wild-type TetR, (B) with TetR-Q38A and (C) with TetR-R28A. (D) ITC measurements showing the titration of the RNA aptamer with wild-type TetR, (E) titration with TetR-Q38A and (F) titration with TetR-R28A. (G) ITC measurements showing the titration of double-stranded tetO operator DNA with wild-type TetR, (H) with TetR-Q38A and (I) with TetR-R28A.